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Hypertension pp 275-296 | Cite as

Proteomic Approaches in the Analysis of Hypertension

  • Soren Naaby-Hansen
  • Gayathri D.
  • Claire Hastie
  • Piers Gallney
  • Rainer Cramer
Part of the Methods In Molecular Medicine™ book series (MIMM, volume 108)

Abstract

The completion of the genomic sequence and the definition of the genes provide a wealth of data to interpret cellular protein expression patterns and relate them to protein function. Proteomics is the large-scale study of proteins in the post-genomic era, aimed at identifying and characterizing protein expression, function, posttranslational modification, regulation, trafficking, interaction and structure, and their perturbation by disease and drug action. The multigenetic background and essentially unknown etiology of hypertension, makes this main killer a prime candidate for proteomic analysis. The classical proteomic approaches are based on twodimensional gel electrophoretic protein separation and their subsequent identification and characterization by mass spectrometry analysis. However, expression level analysis may not reflect the functional state of proteins and is biased towards long-lived abundant proteins. This review describes a variety of techniques that can be used to identify low-abundance proteins that may be of more functional interest. The modification of classical two-dimensional electrophoresis in order to study post-translational modifications, e.g., phosphorylation, is also discussed.

Key Words

Proteomics two-dimensional electrophoresis (2-DE) mass spectrometry difference gel electrophoresis (DIGE) cell surface/metabolic labeling phosphorylation cell signalling 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Soren Naaby-Hansen
    • 1
  • Gayathri D.
    • 2
  • Claire Hastie
    • 2
  • Piers Gallney
    • 3
  • Rainer Cramer
    • 3
  1. 1.Ludwig Institute for Cancer Research and Department of Biochemistry and Molecular BiologyRoyal Free and University College London Medical SchoolLondonUK
  2. 2.Ludwig Institute for Cancer ResearchRoyal Free and University College London Medical SchoolLondonUK
  3. 3.Ludwig Institute for Cancer ResearchRoyal Free and University College London Medical SchoolLondonUK

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