Hypertension pp 149-158 | Cite as

Single-Strand Conformational Polymorphism Analysis

Basic Principles and Routine Practice
  • Haidong Zhu 
Part of the Methods In Molecular Medicine™ book series (MIMM, volume 108)

Abstract

Single-strand conformational polymorphism (SSCP) analysis is a simple and sensitive technique for mutation detection and genotyping. The principle of SSCP analysis is based on the fact that single-stranded DNA has a defined conformation. Altered conformation due to a single base change in the sequence can cause single-stranded DNA to migrate differently under nondenaturing electrophoresis conditions. Therefore wild-type and mutant DNA samples display different band patterns.

Key Words

Single-strand conformational polymorphism (SSCP) silver staining mutationdiscovery 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Haidong Zhu 
    • 1
  1. 1.Georgia Prevention InstituteMedical College of GeorgiaAugusta

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