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Design of a Herpes Simplex Virus Type 2 Long Oligonucleotide-Based Microarray

Global Analysis of HSV-2 Transcript Abundance During Productive Infection

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Book cover DNA Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 292))

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Abstract

The design and construction of a long (75-mer) oligonucleotide-based DNA microarray for herpes simplex virus type 2 transcripts is described. This array is utilized to generate an analysis of HSV-2 transcript abundance as a function of conditions of infection of human cells, and global patterns of HSV-2 transcript abundance are compared with those for HSV-1. General similarities in patterns along with notable differences in specific details are noted. These results reveal a marked conservation in the program of gene activity between phenotypically diverged strains.

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Author information

Authors and Affiliations

  1. Department of Molecular Biology and Biochemistry and Center for Virus Research, University of California, Irvine, Irvine, CA

    J. S. Aguilar

  2. Scottish Centre for Genomic Technology and Informatics, University of Edinburgh Medical School, The Chancellor’s Building, Little France Crescent, Edinburgh, UK

    Peter Ghazal

  3. Department of Molecular Biology and Biochemistry, Institute for Genomics and Bioinformatics, and Center for Virus Research, University of California, Irvine, CA

    Edward K. Wagner

Authors
  1. J. S. Aguilar
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  2. Peter Ghazal
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  3. Edward K. Wagner
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Editor information

Editors and Affiliations

  1. The Wistar Institute, Philadelphia, PA

    Paul M. Lieberman

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© 2005 Humana Press Inc.

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Aguilar, J.S., Ghazal, P., Wagner, E.K. (2005). Design of a Herpes Simplex Virus Type 2 Long Oligonucleotide-Based Microarray. In: Lieberman, P.M. (eds) DNA Viruses. Methods in Molecular Biology, vol 292. Humana Press. https://doi.org/10.1385/1-59259-848-X:423

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  • DOI: https://doi.org/10.1385/1-59259-848-X:423

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-353-4

  • Online ISBN: 978-1-59259-848-9

  • eBook Packages: Springer Protocols

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