Abstract
This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Banker, G. and Goslin, K. (eds.) (1998) Culturing Nerve Cells, 2nd ed. MIT Press, Cambridge, MA.
Fedoroff, S. and Richardson, A. (eds.) (2001) Protocols for Neural Cell Culture, 3rd ed. Humana, Totowa, NJ.
Tomishima, M. J., Smith, G. A., and Enquist, L. W. (2001) Sorting and transport of alpha herpesviruses in axons. Traffic 2, 429–436.
Tischler, A. S. and Greene, L. A. (1975) Nerve growth factor-induced process formation by cultured rat pheochromocytoma cells. Nature 258, 341–342.
Greene, L. A., Burstein, D. E., and Black, M. M. (1982) The role of transcriptiondependent priming in nerve growth factor promoted neurite outgrowth. Dev. Biol. 91, 305–316.
Greene, L. A., Farinelli, S. E., Cunningham, M. E., and Park, D. S. (1998) Culture and experimental use of the PC12 rat pheochromocytoma cell line, in Culturing Nerve Cells, 2nd ed. (Banker, G. and Goslin, K., eds.), MIT Press, Cambridge, MA, pp. 161–188.
Mobley, W. C, Schenker, A., and Shooter, E. M. (1976) Characterization and isolation of proteolytically modified nerve growth factor. Biochemistry 15, 5543–5552.
Greene, L. A. (1978) Nerve growth factor prevents the death and stimulates the neuronal differentiation of PC12 pheochromocytoma cells in serum-free medium. J. Cell. Biol. 78, 747–755.
Dichter, M. A., Tischler, A. S., and Greene, L. A. (1977) Nerve growth factorinduced increase in electrical excitability and acetylcholine sensitivity of a rat pheochromocytoma cell line. Nature 268, 501–504.
Su, Y. H., Moxley, M., Kejariwal, R., Mehta, A., Fraser, N. W., and Block, T. M. (2000) The HSV-1 genome in quiescently infected NGF differentiated PC12 cells cannot be stimulated by HSV superinfection. J. Neurovirol. 6, 341–349.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Hean Ch’ng, T., Flood, E.A., Enquist, L.W. (2005). Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection. In: Lieberman, P.M. (eds) DNA Viruses. Methods in Molecular Biology, vol 292. Humana Press. https://doi.org/10.1385/1-59259-848-X:299
Download citation
DOI: https://doi.org/10.1385/1-59259-848-X:299
Publisher Name: Humana Press
Print ISBN: 978-1-58829-353-4
Online ISBN: 978-1-59259-848-9
eBook Packages: Springer Protocols