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DNA Viruses pp 73-81 | Cite as

Reporter Cell Lines for the Detection of Herpes Simplex Viruses

  • Szu-Hao Kung
Part of the Methods in Molecular Biology book series (MIMB, volume 292)

Abstract

Virus culture has played significant roles in basic and clinical virology, with a number of advantages that cannot be attainable by modern molecular techniques. However, virus culture is generally a slower process, as it inevitably takes the period of a full replication cycle of a given virus. A genetically modified cell culture with a virus-inducible marker is described here, using a frequently isolated DNA virus (herpes simplex virus) as a model. The assay system relies on expression of the reporter gene driven by a specific viral promoter that is triggered early in the course of viral infection. The reporter gene employed was green fluorescent protein (GFP) or secreted alkaline phosphatase (SEAP), whose assays offer real-time detection or quantification, respectively. This cell-based assay is simple, rapid, sensitive, specific, and quantitative and serves as a phenotypic method for determination of antiviral susceptibilities.

Key Words

Herpes simplex virus reporter cell line reporter gene, green fluorescent protein secreted alkaline phosphatase acyclovir antiviral susceptibility testing ICP10 promoter 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Szu-Hao Kung
    • 1
  1. 1.Faculty of Medical Technology and Institute of Biotechnology in MedicineNational Yang-Ming UniversityTaipei, Taiwan, Republic of China

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