Abstract
Modification of Xanthophyllomyces dendrorhous toward a higher carotenoid synthesis will make the exploitation of X. dendrorhous as a natural source for astaxanthin more competitive. By recombinant DNA technology, the X. dendrorhous isopentenyl-pyrophosphate isomerase-encoding gene (idi) was inserted in a X. dendrorhous expression cassette that directs expression by the endogenous glyceraldehyde-3-phosphate dehydrogenase gene expression signals. X. dendrorhous transformation vector pPR2TN was used to deliver this expression cassette, by means of electroporation, into the ribosomal DNA of X. dendrorhous strain CBS 6938, which was confirmed by Southern analysis. High-performance liquid chromatography analysis of the carotenoids produced by idi-overexpressing transformant strains indicated a lower overall carotenoid formation when compared to the control strain. This finding is in contrast to that observed in bacterial systems, where enhanced expression of the idi gene increases the carotenoid content.
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Visser, H., Sandmann, G., Verdoes, J.C. (2005). Xanthophylls in Fungi. In: Barredo, JL. (eds) Microbial Processes and Products. Methods in Biotechnology, vol 18. Humana Press. https://doi.org/10.1385/1-59259-847-1:257
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DOI: https://doi.org/10.1385/1-59259-847-1:257
Publisher Name: Humana Press
Print ISBN: 978-1-58829-548-4
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