Abstract
Nondigestible oligosaccharides (NDOs), namely prebiotic oligosaccharides, are functional food ingredients that possess properties that are beneficial to the health of consumers. These include noncariogenicity, a low calorific value, and the ability to stimulate the growth of beneficial bacteria in the colon. Fructooligosaccharides (FOS) represent one of the major classes of oligosaccharides in terms of their production volume. They are manufactured by two different processes, which result in slightly different end products. First, FOS are produced by controlled hydrolysis of inulin. Secondly, FOS can be produced from sucrose by using the transfructosylat-ing activity of the enzyme β-fructofuranosidase (EC 3.2.1.26) at high concentrations of the starting material. This chapter summarizes the methods used for the detection and characterization of enzymes of fungal origin with fructosyltransferase activity and the analytical methods utilized to identify the oligosaccharides produced.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gibson, G. R. and Roberfroid, M. B. (1995) Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J. Nutr. 125, 1401–1412.
van Loo, J., Cummings, J., Delzenne, N., Englyst, H., Franck, A., Hopkins, M., et al. (1999) Functional food properties of non-digestible oligosaccharide: a consen-sus report from the ENDO project (DGXIIAIRII-CT94-1095). British J. Nutr. 81, 121–132.
Hidaka, H, Hirayama, M., and Yamada, K. (1991) Fructooligosaccharides: enzymatic preparation and biofunctions. J. Carbohydrate Chem. 10, 509–522.
Yun, J. W. (1996) Fructooligosaccharides: Occurrence, preparation and application. Enzyme Microb. Technol. 19, 107–117.
Hidaka, H., Hirayama, M., and Sumi, N. A. (1988) A fructooligosaccharide-pro-ducing enzyme from Aspergillus niger ATCC20611. Agric. Biol. Chem. 52, 1181–1187.
Cairns, A. J. (1987) Colorimetric microtiter plate assay of glucose and fructose by enzyme linked formazan production: applicability of the measurement of fructosyl transferase activity in higher plants. Anal. Biochem. 167, 270–278.
Heyer, A. G. and Wendenburg, R. (2001) Gene cloning and functional characterization by heterologous expression of the fructosyltransferase of Aspergillus sydowi IAM 2544. Appl. Environ. Microbiol. 67, 363–370.
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Boddy, L. M., Berges, T., Barreau, C., Vainstein, M. H., Dobson, M. J., Balance, D. J., and Peberdy, J. F. (1993) Purification and characterization of an Aspergillus niger invertase and its DNA sequence. Curr. Genet. 24, 60–66.
Somiari, R. I., Brzeski, H., Tate, R., Bielecki, S., and Polak, J. (1997) Cloning and sequencing of an Aspergillus niger gene coding for β-fructofuranosidase. Biotechnol. Lett. 19, 1243–1247.
Yanai, K., Nakene, A., Kawate, A., and Hirayama, M. (2001) Molecular cloning and characterization of the fructooligosaccharide-producing β-fructofuranosidase gene from Aspergillus niger ATCC 20611. Biosci. Biotechnol. Biochem. 65, 766–773.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Bañuelos, O., Carmen Ronchel, M.d., Adrio, J.L., Velasco, J. (2005). Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides. In: Barredo, J.L. (eds) Microbial Enzymes and Biotransformations. Methods in Biotechnology, vol 17. Humana Press. https://doi.org/10.1385/1-59259-846-3:105
Download citation
DOI: https://doi.org/10.1385/1-59259-846-3:105
Publisher Name: Humana Press
Print ISBN: 978-1-58829-253-7
Online ISBN: 978-1-59259-846-5
eBook Packages: Springer Protocols