Abstract
Cell culture is an invaluable tool for investigators in numerous fields. It facilitates analysis of biological properties and processes that are not readily accessible at the level of the intact organism. Successful maintenance of cells in culture, whether primary or immortalized, requires knowledge and practice of a few essential techniques. The purpose of this chapter is to explain the basic principles of cell culture using the maintenance of a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples. Procedures for thawing, culture, determination of cell numbers and viability, and cryopreservation are described.
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References
Freshney, R. I. (ed.) (2000) Culture of Animal Cells. A Manual of Basic Techniques, 2nd ed., Wiley, New York.
Celis, J. F. (ed.) (1998) Cell Biology: A Laboratory Handbook, 2nd ed., Academic, New York.
Davis, J. M. (ed.) (2002) Basic Cell Culture, 2nd ed., IRL, Oxford.
Bonifacino, J. S., Dasso, M., Harford, J. B., Lippincott-Schwartz, J:, and Yamada, K. M. (eds.) (2000) Current Protocols in Cell Biology, Wiley, New York.
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© 2005 Humana Press Inc., Totowa, NJ
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Helgason, C.D. (2005). Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line. In: Helgason, C.D., Miller, C.L. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology™, vol 290. Humana Press. https://doi.org/10.1385/1-59259-838-2:001
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DOI: https://doi.org/10.1385/1-59259-838-2:001
Publisher Name: Humana Press
Print ISBN: 978-1-58829-284-1
Online ISBN: 978-1-59259-838-0
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