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Catalytic Function and Expression of Glutathione Transferase Zeta

  • Philip G. Board
  • M. W. Anders
  • Anneke C. Blackburn
Protocol
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

The zeta class of glutathione S-transferases (GSTZ) is one of the most recently discovered soluble GST classes and has proved to be of considerable interest because of its contribution to the catabolism of phenylalanine and tyrosine and its role in α-halo acid metabolism. GSTZ was originally discovered as a result of a bioinformatic approach to gene discovery in the mid-1990s. This approach and others have also led to the discovery of several polymorphic forms of GSTZ. This chapter summarizes methods and approaches that have been used to express recombinant GSTZ, determine its crystal structure, measure its activity and characterize its kinetic properties, and study the function and importance of GSTZ in the metabolism of dichloroacetate (DCA), which is of great toxicological and public health interest. DCA is a multisite toxicant and carcinogen that is a byproduct of water chlorination and is a breakdown product of the industrial chemical and environmental contaminant trichloroethylene.

Key Words

Bioinformatics BLAST programs dichloroacetic acid expressed sequence tag fumarylaceto-acetate hydrolase gene knockout glutathione S-transferase-zeta glyoxylic acid α-halo acids leukotrienes maleylacetoacetate isomerase polymorphisms single nucleotide polymorphisms 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Philip G. Board
    • 1
  • M. W. Anders
    • 2
  • Anneke C. Blackburn
    • 1
  1. 1.Molecular Genetics, Division of Molecular Bioscience, Institute of Advanced Studies, The John Curtin School of Medical ResearchAustralian National UniversityCanberraAustralia
  2. 2.Department of Pharmacology and PhysiologyUniversity of RochesterRochester

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