Abstract
In epidermal cells desmosomes represent major sites of basolateral cell-cell contacts that play an important role for epidermal homeostasis. Excess or damaged cells are often removed by apoptosis. To release apoptotic cells from the tissue, intercellular contacts have to be broken. Here detailed protocols to analyze desmosomal proteins in apoptotic cells are described.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Krammer, P. H. (2000) CD95’s deadly mission in the immune system. Nature 407, 789–795.
Ferri, K. F and Kroemer, G. (2001) Organelle-specific initiation of cell death pathways. Nat. Cell Biol. 3, E–255–E–263.
Meier, P., Finch, A., and Evan, G. (2000) Apoptosis in development. Nature 407, 796–801.
Hathaway, T. R. and Kuechle, M. K. (2002) Apoptosis and cutaneous biology. Adv. Dermatol. 18, 287–311.
Earnshaw, W. C, Martins, L. M., and Kaufmann, S. H. (1999) Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu. Rev. Biochem. 68, 383–424.
Steinhusen, U., Weiske, J., Badock, V., Tauber, R., Bommert, K., and Huber, O. (2001) Cleavage and shedding of E-cadherin after induction of apoptosis. J. Biol. Chem. 276, 4972–4980.
Weiske, J., Schoeneberg, T., Schroeder, W., Hatzfeld, M., Tauber, R., and Huber, O. (2001) The fate of desmosomal proteins in apototic cells. J. Biol. Chem. 276, 41,175–41,181.
Garrod, D. R., Merritt, A. J., and Nie, Z. (2002) Desmosomal cadherins. Curr. Opin. Cell Biol. 14, 537–545.
Garrod, D. R., Merritt, A. J., and Nie, Z. (2002) Desmosomal adhesion: structural basis, molecular mechanism and regulation. Mol. Membr. Biol. 19, 81–94.
Green, K. J. and Gaudry, C. A. (2000) Are desmosomes more than tethers for intermediate filaments? Nat. Rev. Mol. Cell Biol. 1, 208–216.
Huber, O. (2003) Structure and function of desmosomal proteins and their role in development and disease. Cell Mol. Life Sci. 60, 1872–1890.
Huber, O. and Huber-Wunderlich, M. (2000) Recombinant proteins, in Journal of chromatography library 61, Protein liquid chromatography (Kastner, M., ed.), Elsevier Science B. V., Amsterdam, The Netherlands, pp. 557–586.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Weiske, J., Huber, O. (2005). Fate of Desmosomal Proteins in Apoptotic Epidermal Cells. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology™, vol 289. Humana Press. https://doi.org/10.1385/1-59259-830-7:175
Download citation
DOI: https://doi.org/10.1385/1-59259-830-7:175
Publisher Name: Humana Press
Print ISBN: 978-1-58829-267-4
Online ISBN: 978-1-59259-830-4
eBook Packages: Springer Protocols