Abstract
Reverse transcription polymerase chain reaction is used for the semiquantitative analysis of epidermal gene expression, particularly when immunolocalization is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction method to analyze gene expression both in vivo and in vitro.
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Turksen, K., and Troy, T. C. (2002) Permeability barrier dysfunction in transgenic mice over-expressing claudin 6. Development 129, 1775–1784.
Turksen, K. and Troy, T. C. (2003) Overexpression of the calcium sensing receptor accelerates epidermal differentiation and permeability barrier formation in vivo. Mech. Dev. 120, 733–744.
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© 2005 Humana Press Inc.
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Troy, TC., Man-Kit Cheung, R., Turksen, K. (2005). Epidermal Cell Analysis by RT-PCR. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology™, vol 289. Humana Press. https://doi.org/10.1385/1-59259-830-7:121
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DOI: https://doi.org/10.1385/1-59259-830-7:121
Publisher Name: Humana Press
Print ISBN: 978-1-58829-267-4
Online ISBN: 978-1-59259-830-4
eBook Packages: Springer Protocols