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Keratinocyte Culture in the Absence of Substrate Attachment

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Epidermal Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 289))

Abstract

This chapter deals with experimental protocols and considerations related to the culture of epithelial cells under anchorage-independent conditions in liquid media. This technique has proven to be a powerful tool in studying the effects of loss of extracellular matrix interaction on crucial aspects of epithelial cell biology. Specifically, examining cells in the absence of substrate attachment, as described in this chapter, will allow the investigator to study the effect of growth factors independently of cell adhesion. Several methods are discussed relating to the preparation of tissue culture plates for suspension cultures and to the choice of a suitable cell culture medium.

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Author information

Authors and Affiliations

  1. SymbioTec GmbH, Saarbrücken, Germany

    Monika Jost

  2. Department of Dermatology and Cutaneous Biology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA

    Ulrich Rodeck

Authors
  1. Monika Jost
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  2. Ulrich Rodeck
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Editor information

Editors and Affiliations

  1. Ottawa Health Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2005 Humana Press Inc.

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Jost, M., Rodeck, U. (2005). Keratinocyte Culture in the Absence of Substrate Attachment. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology™, vol 289. Humana Press. https://doi.org/10.1385/1-59259-830-7:023

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  • DOI: https://doi.org/10.1385/1-59259-830-7:023

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-267-4

  • Online ISBN: 978-1-59259-830-4

  • eBook Packages: Springer Protocols

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