Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morphogenesis

  • Mariko Kashiwagi
  • Nam-ho Huh
Part of the Methods in Molecular Biology™ book series (MIMB, volume 289)


In this chapter, we describe an organ culture method that allows skin tissues isolated from 12.5- or 13.5-d postcoitus mouse embryos to develop in a manner histologically and temporally similar to the process in vivo. In this culture system, epidermal growth factor specifically and completely inhibited the hair follicle formation with marginal effects on interfollicular epidermis. Using an adenovirus vector, one can transduce a gene into the epidermal and dermal cell layers without appreciable toxicity.

Key Words

Organ culture skin development adenovirus 


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Mariko Kashiwagi
    • 1
  • Nam-ho Huh
    • 2
  1. 1.Institute of Molecular OncologyShowa UniversityTokyoJapan
  2. 2.Department of Cell BiologyOkayama University Graduate School of Medicine and DentistryOkayamaJapan

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