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Inhibition of Histone Deacetylases

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Book cover Epigenetics Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 287))

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Abstract

Reversible histone acetylation, governed dynamically by histone acetyltransferases (HATs) and histone deacetylases (HDACs), plays a pivotal role in regulation of gene expression through remodeling chromatin structure. Manipulation of the equilibrium between acetylation and deacetylation of histones by specific HDAC inhibitors is thus a useful tool to study functional role(s) for histone hyper-/hypoacetylation in controlling gene transcription and many other cellular activities. By using the trans-activating effect of trichostatin A (TSA), a widely used HDAC inhibitor, on the telomerase reverse transcriptase (hTERT) gene as an example, we summarize various aspects of HDAC inhibitors and provide a general strategy for their in vitro application in studies of gene regulation.

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Author information

Authors and Affiliations

  1. Department of Medicine, Division of Hematology, Karolinska Hospital, Stockholm, Sweden

    Cheng Liu & Dawei Xu

Authors
  1. Cheng Liu
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  2. Dawei Xu
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Editor information

Editors and Affiliations

  1. Department of Biology, University of Alabama, Birmingham, AL

    Trygve O. Tollefsbol

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© 2004 Humana Press Inc., Totowa, NJ

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Liu, C., Xu, D. (2004). Inhibition of Histone Deacetylases. In: Tollefsbol, T.O. (eds) Epigenetics Protocols. Methods in Molecular Biology™, vol 287. Humana Press. https://doi.org/10.1385/1-59259-828-5:087

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  • DOI: https://doi.org/10.1385/1-59259-828-5:087

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-336-7

  • Online ISBN: 978-1-59259-828-1

  • eBook Packages: Springer Protocols

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