Abstract
Active genes in eukaryotic genomes are typically found in open, nuclease-sensitive regions of chromatin. This chapter presents an overview of the techniques used to assay restriction endonuclease cleavage of chromosomal DNA as an approach to assess general accessibility at a genomic region of interest. We describe protocols to (1) prepare nuclei templates, (2) treat chromosomal DNA with a restriction enzyme(s), and (3) visualize and quantify chromosomal cleavage(s), with an emphasis on ligation-mediated (LM) PCR techniques.
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© 2004 Humana Press Inc., Totowa, NJ
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Hempel, W.M., Ferrier, P. (2004). Restriction Endonuclease Accessibility as a Determinant of Altered Chromatin Structure. In: Tollefsbol, T.O. (eds) Epigenetics Protocols. Methods in Molecular Biology™, vol 287. Humana Press. https://doi.org/10.1385/1-59259-828-5:053
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DOI: https://doi.org/10.1385/1-59259-828-5:053
Publisher Name: Humana Press
Print ISBN: 978-1-58829-336-7
Online ISBN: 978-1-59259-828-1
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