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Transgenic Plants: Methods and Protocols pp 191–201Cite as

Selectable Markers: Antibiotic and Herbicide Resistance

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 286))

Summary

The low efficiencies of most plant transformation methods necessitate the use of selectable marker genes to identify those cells that successfully integrate and express transferred DNA. Genes conferring resistance to various antibiotics or herbicides are commonly used in laboratory transformation research. They encode proteins that detoxify corresponding selection agents and allow the preferential growth of transformed cells. This chapter describes the application of two selection systems on the transformation of wheat. One is based on the nptII gene and corresponding aminoglycoside antibiotics, the other is based on the bar gene and corresponding glufosinate ammonium herbicides.

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Author information

Authors and Affiliations

  1. Crop Performance and Improvement Department, Rothamsted Research, Hertfordshire, UK

    Julia L. Goodwin, Gabriela M. Pastori & Huw D. Jones

  2. Plant Sciences Division, School of Biosciences, University of Nottingham, Loughborough, UK

    Michael R. Davey

Authors
  1. Julia L. Goodwin
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  2. Gabriela M. Pastori
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  3. Michael R. Davey
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  4. Huw D. Jones
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Editor information

Editors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain

    Leandro Peña

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© 2005 Humana Press Inc.

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Cite this protocol

Goodwin, J.L., Pastori, G.M., Davey, M.R., Jones, H.D. (2005). Selectable Markers: Antibiotic and Herbicide Resistance. In: Peña, L. (eds) Transgenic Plants: Methods and Protocols. Methods in Molecular Biology™, vol 286. Humana Press. https://doi.org/10.1385/1-59259-827-7:191

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  • DOI: https://doi.org/10.1385/1-59259-827-7:191

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-263-6

  • Online ISBN: 978-1-59259-827-4

  • eBook Packages: Springer Protocols

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