Hematopoietic Development of Human Embryonic Stem Cells in Culture

  • Xinghui Tian
  • Dan S. Kaufman
Part of the Methods in Molecular Medicine book series (MIMM, volume 105)


The isolation of embryonic stem (ES) cells from human preimplantation blastocytes creates an exciting new starting point to analyze the earliest stages of human blood development. This chapter describes two methods for the promotion of hematopoietic differentiation of human ES cells: stromal cell co-culture and embryoid body formation. Better understanding of basic human hematopoiesis through the study of human ES cells will likely have future therapeutic benefits.

Key Words

Embryonic stem cells hematopoiesis embryoid body stromal cell differentiation hematopoietic stem cell 


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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Xinghui Tian
    • 1
  • Dan S. Kaufman
    • 1
  1. 1.Stem Cell Institute and Department of MedicineUniversity of MinnesotaMinneapolis

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