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Oligonucleotide Synthesis pp 305–318Cite as

Nonenzymatic Template-Directed RNA Synthesis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 288))

Abstract

The aim of this chapter is to provide reliable protocols for the study of nonenzymatic oligomerization reactions of ribonucleic acid and its analogs. Traditional radioactive labels are replaced by fluorescent dyes. Product analysis is either based on reversed-phase high-pressure (performance) liquid chromatography or on gel electrophoresis with on-line detection applying a deoxyribonucleic acid sequencer. Three examples of primer extensions are given, one of which demonstrates how the reaction of several primers may be monitored simultaneously.

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Author information

Authors and Affiliations

  1. Department of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Frankfurt am Main, Germany

    Marcus Hey & Michael Göel

Authors
  1. Marcus Hey
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  2. Michael Göel
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Editor information

Editors and Affiliations

  1. Rega Institute, Medicinal Chemistry Lab, Katholieke Universiteit Leuven, Leuven, Belgium

    Piet Herdewijn

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© 2005 Humana Press Inc.

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Hey, M., Göel, M. (2005). Nonenzymatic Template-Directed RNA Synthesis. In: Herdewijn, P. (eds) Oligonucleotide Synthesis. Methods in Molecular Biology, vol 288. Humana Press. https://doi.org/10.1385/1-59259-823-4:305

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  • DOI: https://doi.org/10.1385/1-59259-823-4:305

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-233-9

  • Online ISBN: 978-1-59259-823-6

  • eBook Packages: Springer Protocols

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