Synthesis of DNA Using a New Two-Step Cycle

  • Douglas J. Dellinger
  • Jason R. Betley
  • Tadeusz K. Wyrzykiewicz
  • Marvin H. Caruthers
Part of the Methods in Molecular Biology book series (MIMB, volume 288)


For the first time a new, two-step method is described for synthesizing deoxyribonucleic acid. The approach uses 5′-carbonate protected 2′-deoxynucleoside-3′-phosphoramidites as synthons and a peroxy anion buffer that removes the carbonate protecting group and oxidizes the internucleotide linkage. Following synthesis via this two-step cycle, oligomers are isolated by standard procedures.

Key Words

Two-step synthesis cycle 5′-carbonate protection peroxy anion deprotection and oxidation 


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Douglas J. Dellinger
    • 1
  • Jason R. Betley
    • 2
  • Tadeusz K. Wyrzykiewicz
    • 3
  • Marvin H. Caruthers
    • 4
    • 5
  1. 1.Agilent LaboratoriesFort Collins
  2. 2.EmmbrookUK
  3. 3.Isis PharmaceuticalsCarlsbad
  4. 4.Department of Chemistry and BiochemistryUniversity of Colorado at BoulderBoulder
  5. 5.Agilent LaboratoriesPalo Alto

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