Synthesis of DNA Using a New Two-Step Cycle
For the first time a new, two-step method is described for synthesizing deoxyribonucleic acid. The approach uses 5′-carbonate protected 2′-deoxynucleoside-3′-phosphoramidites as synthons and a peroxy anion buffer that removes the carbonate protecting group and oxidizes the internucleotide linkage. Following synthesis via this two-step cycle, oligomers are isolated by standard procedures.
Key WordsTwo-step synthesis cycle 5′-carbonate protection peroxy anion deprotection and oxidation
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