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Single and Double Colloidal Gold Labeling in Postembedding Immunoelectron Microscopy

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Cell Cycle Control and Dysregulation Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 285))

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Abstract

Immunocytochemistry is the most diffuse technique to visualize and localize specific biochemical components in cell compartments and tissues. With this method the antigens are tagged by antibodies that can be visualized with appropriate markers attached directly (direct method) or indirectly (indirect method) to them. The colloidal gold marker system (1) is the most widespread immunoelectron microscopy label for postembedding immunocytochemistry. Sections of embedded tissue are incubated with a primary antibody against a particular antigen, which is exposed on the surface of the section, and then with a secondary antibody conjugated to colloidal gold particles of a particular size (indirect method). It is possible to enlarge the colloidal gold size with silver enhancement. This technique is ideal for labeling both intracellular and surface antigens and it can be used for multiple labeling experiments and quantitative studies (2).

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References

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Author information

Authors and Affiliations

  1. Institute of Organ Transplant and Immunocytology, Bologna Unit, National Council of Research CNR), c/o IOR, Bologna, Italy

    Nicoletta Zini, Caterina Cinti & Nadir Mario Maraldi

  2. Laboratory of Cell Biology and Electron Microscopy, IOR, Institute of Histology and General Embryology, University of Bologna, Bologna, Italy

    Liliana Solimando

  3. Laboratory of Cell Biology and Electron Microscopy, IOR, Department of Anatomical Sciences, University of Bologna, Bologna, Italy

    Nadir Mario Maraldi

Authors
  1. Nicoletta Zini
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  2. Liliana Solimando
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  3. Caterina Cinti
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  4. Nadir Mario Maraldi
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Editor information

Editors and Affiliations

  1. Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, PA

    Antonio Giordano

  2. Department of Neurosurgery, Thomas Jefferson University, Philadelphia, PA

    Gaetano Romano

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© 2004 Humana Press Inc.

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Zini, N., Solimando, L., Cinti, C., Maraldi, N.M. (2004). Single and Double Colloidal Gold Labeling in Postembedding Immunoelectron Microscopy. In: Giordano, A., Romano, G. (eds) Cell Cycle Control and Dysregulation Protocols. Methods in Molecular Biology™, vol 285. Humana Press. https://doi.org/10.1385/1-59259-822-6:161

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  • DOI: https://doi.org/10.1385/1-59259-822-6:161

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-949-0

  • Online ISBN: 978-1-59259-822-9

  • eBook Packages: Springer Protocols

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