Abstract
Apoptosis plays a critical role in important biological processes such as morphogenesis, tissue homeostasis, and elimination of genomic damaged or virally infected cells and of self-reactive clones from the immune system (1). Although apoptosis is important during normal development, its aberrant activation may contribute to a number of diseases, such as AIDS, neurodegenerative disorders, and ischemic injury (2). However, impaired apoptosis may be a significant factor in the etiology of diseases like cancer, autoimmune disorders, and viral infections (2).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Steller H. (1995) Mechanisms and genes of cellular suicide (review). Science 267, 1445–1449.
Thompson, C. B. (1995) Apoptosis in the pathogenesis and treatment of disease (review). Science 267, 1456–1462.
Wyllie, A. H., Kerr, J. F. R., and Currie, A. R. (1980) Cell death: the significance of apoptosis. Int. Rev. Cytol. 68, 251–306.
Arends, M. J. and Wyllie, A. H. (1991) Apoptosis: mechanisms and roles in pathology. Int. Rev. Exp. Pathol. 32, 223–254.
Falcieri, E., Zamai, L., Santi, S., Cinti, C., Gobbi, P., Bosco, D., et al. (1994) The behaviour of nuclear domains in the course of apoptosis. Histochemistry 102, 221–231.
Arends, M. J., Morris, R. G., and Wyllie, A. H. (1990) Apoptosis: the role of the endonuclease. Am. J. Pathol. 136, 593–608.
Peitsch, C., Muller, C., and Tschopp, J. (1993) DNA fragmentation during apoptosis is caused by frequent single strand cuts. Nucl. Acid Res. 21, 4206–4209.
Peitsch, M. C., Polzar, B., Stephan, H., Crompton, T., MacDonald, H. R., and Mannhertz, H. G. (1993) Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death). EMBO J. 12, 371–377.
Oberhammer, F., Wilson, J. W., Dive, C., Morris, I. D., Hickman, J. A., Wakeling, A. E., et al. (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 Kb fragments prior or in absence of internucleosomal fragmentation. EMBO J. 12, 3679–3684.
Falcieri, E., Suppia, L., Di Baldassarre, A., Mariani, A. R., Cinti, C., Columbaro, M., et al. (1996) Different approaches to the study of apoptosis. Scan Microsc. 10, 227–237.
Lucchetti, F., Mariani, A. R., Columbaro, M., Di Baldassarre, A., Cinti, C., and Falcieri, E. (1998) Apoptotic pathways depend on the target enzymatic activity and not on the triggering agent. Scanning 21, 29–35.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc.
About this protocol
Cite this protocol
Trimarchi, C., La Sala, D., Zamparelli, A., Cinti, C. (2004). Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation. In: Giordano, A., Romano, G. (eds) Cell Cycle Control and Dysregulation Protocols. Methods in Molecular Biology™, vol 285. Humana Press. https://doi.org/10.1385/1-59259-822-6:113
Download citation
DOI: https://doi.org/10.1385/1-59259-822-6:113
Publisher Name: Humana Press
Print ISBN: 978-0-89603-949-0
Online ISBN: 978-1-59259-822-9
eBook Packages: Springer Protocols