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Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays

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Book cover Apoptosis Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 282))

Abstract

Natural killer (NK) cells are a crucial component of the immune system. For example, the NK cell-mediated killing of tumor cells is a first line of defense against the development of cancer. Detection and quantification of apoptosis induced in target cells by NK cells is a useful tool for studies of the mechanisms of both immunological protection and pathogenesis. This chapter describes how to do this by using techniques with a broad application to both NK cells and cytotoxic T cells.

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References

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Author information

Authors and Affiliations

  1. CERES Pharmaceuticals Ltd., Medical Oncology, University of Colorado Health Science Center, and Eleanor Roosevelt Institute for Cancer Research, Denver, CO

    Richard C. Duke

Authors
  1. Richard C. Duke
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Editor information

Editors and Affiliations

  1. Institute of Child Health, University College London, London, UK

    Hugh J. M. Brady

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© 2004 Humana Press Inc.

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Duke, R.C. (2004). Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays. In: Brady, H.J.M. (eds) Apoptosis Methods and Protocols. Methods in Molecular Biology, vol 282. Humana Press. https://doi.org/10.1385/1-59259-812-9:043

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  • DOI: https://doi.org/10.1385/1-59259-812-9:043

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-873-8

  • Online ISBN: 978-1-59259-812-0

  • eBook Packages: Springer Protocols

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