Abstract
In situ hybridization allows detection and localization of specific nucleic acid sequences directly within a cell or tissue. We present an in situ hybridization protocol using double-stranded DNA or single-stranded RNA probes labeled with [32P] to localize and visualize the temporal and spatial distribution of cartilage-characteristic mRNAs. Probes labeled with this high-energy isotope provide good resolution at the tissue level with relatively low background; as a result of the probes that can be obtained that have a higher specificity to emulsion activity, very short exposure times are required.
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References
Mallein-Gerin, F., Ruggiero, F., and Garrone, R. (1990) Proteoglycan core protein and type II collagen gene expressions are not correlated with cell shape changes during low density chondrocyte cultures. Differentiation 43, 204–211.
Mallein-Gerin, F., Kosher, R. A., Upholt, W. B., and Tanzer, M. L. (1988) Temporal and spatial analysis of cartilage proteoglycan core protein gene expression during limb development by in situ hybridization. Dev. Biol. 126, 337–345.
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© 2004 Humana Press Inc., Totowa, NJ
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Mallein-Gerin, F., Gouttenoire, J. (2004). Gene Expression Analysis in Cartilage by In Situ Hybridization. In: Sabatini, M., Pastoureau, P., De Ceuninck, F. (eds) Cartilage and Osteoarthritis. Methods in Molecular Medicine™, vol 100. Humana Press. https://doi.org/10.1385/1-59259-810-2:105
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DOI: https://doi.org/10.1385/1-59259-810-2:105
Publisher Name: Humana Press
Print ISBN: 978-1-58829-247-6
Online ISBN: 978-1-59259-810-6
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