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Gene Expression Analysis in Cartilage by In Situ Hybridization

  • Frédéric Mallein-Gerin
  • Jérôme Gouttenoire
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 100)

Abstract

In situ hybridization allows detection and localization of specific nucleic acid sequences directly within a cell or tissue. We present an in situ hybridization protocol using double-stranded DNA or single-stranded RNA probes labeled with [32P] to localize and visualize the temporal and spatial distribution of cartilage-characteristic mRNAs. Probes labeled with this high-energy isotope provide good resolution at the tissue level with relatively low background; as a result of the probes that can be obtained that have a higher specificity to emulsion activity, very short exposure times are required.

Key Words

In situ hybridization gene cartilage chondrocyte mRNA 

References

  1. 1.
    Mallein-Gerin, F., Ruggiero, F., and Garrone, R. (1990) Proteoglycan core protein and type II collagen gene expressions are not correlated with cell shape changes during low density chondrocyte cultures. Differentiation 43, 204–211.CrossRefPubMedGoogle Scholar
  2. 2.
    Mallein-Gerin, F., Kosher, R. A., Upholt, W. B., and Tanzer, M. L. (1988) Temporal and spatial analysis of cartilage proteoglycan core protein gene expression during limb development by in situ hybridization. Dev. Biol. 126, 337–345.CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Frédéric Mallein-Gerin
    • 1
  • Jérôme Gouttenoire
    • 1
  1. 1.Laboratory of Biology and Engineering of Cartilage, CNRS UMR 5086Institut de Biologie et Chimie des ProtéinesLyonFrance

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