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The Anti-DNA Knock-In Model of Systemic Autoimmunity Induced by the Chronic Graft-vs-Host Reaction

  • Protocol
Autoimmunity

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 102))

Summary

The injection of spleen cells from bm12 mice into C57BL/6 recipients induces a chronic graft-vs-host reaction characterized by systemic autoimmunity, including anti-double-stranded DNA (anti-dsDNA) autoantibodies and immune complex-type proliferative glomerulonephritis. If the B6 recipient mice express an anti-DNA Vh site-directed transgene, the repertoire is skewed even more toward the anti-DNA response. Over a period of several weeks, high titers of serum anti-DNA antibodies appear and the mice develop renal damage. This permits the examination of the role of somatic immunoglobulin genetics and B-cell tolerance in a model of systemic lupus erythematosus.

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Author information

Authors and Affiliations

  1. Division of Rheumatology, Department of Medicine, University of Pennsylvania, Philadelphia, PA

    Robert Eisenberg & Arpita Choudhury

Authors
  1. Robert Eisenberg
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  2. Arpita Choudhury
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Editor information

Editors and Affiliations

  1. Departments of Medicine, Microbiology, and Immunology, State University of New York, Upstate Medical University, College of Medicine, Syracuse, NY

    Andras Perl MD, PhD

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© 2004 Humana Press Inc., Totowa, NJ

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Eisenberg, R., Choudhury, A. (2004). The Anti-DNA Knock-In Model of Systemic Autoimmunity Induced by the Chronic Graft-vs-Host Reaction. In: Perl, A. (eds) Autoimmunity. Methods in Molecular Medicine™, vol 102. Humana Press. https://doi.org/10.1385/1-59259-805-6:273

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  • DOI: https://doi.org/10.1385/1-59259-805-6:273

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-231-5

  • Online ISBN: 978-1-59259-805-2

  • eBook Packages: Springer Protocols

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