Abstract
Plastocyanin and cytochrome c 6 are widely distributed over the oxygen-evolving photosynthetic organisms. The two proteins are functionally equivalent, but strongly differ in their global electrostatic charge. In fact, they are acidic in eukaryotes, but either neutral or basic in cyanobacteria. The difference in their electrostatic features is a critical factor in designing the purification procedure, which must be modified and adapted accordingly. This chapter reports the methods for producing (including cell cultures), isolating and purifying plastocyanin and cytochrome c 6—which greatly differ in their isoelectric point—from a number of eukaryotic and prokaryotic organisms.
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© 2004 Humana Press Inc., Totowa, NJ
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Navarro, J.A., Hervás, M., De la Rosa, M.A. (2004). Purification of Plastocyanin and Cytochrome c 6 From Plants, Green Algae, and Cyanobacteria. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods In Molecular Biology™, vol 274. Humana Press. https://doi.org/10.1385/1-59259-799-8:079
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DOI: https://doi.org/10.1385/1-59259-799-8:079
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