Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II

  • Grzegorz Jackowski
Part of the Methods In Molecular Biology™ book series (MIMB, volume 274)

Abstract

The protocols regarding separation and purification of LHCII subcomplexes by non-denaturing isoelectric focusing (ndIEF), confirmation of trimeric nature of the subcomplexes, and establishing their polypeptide composition are described below.

Key Words

Circular dichroism denaturing isoelectrofocusing electrophoresis immunoblotting light-harvesting chlorophyll a/b-protein complex of photosystem II (LHCII) monomer nondenaturing isoelectrofocusing polypeptide stacked thylakoid membrane subcomplex trimer, Sultracentrifugation 

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Grzegorz Jackowski
    • 1
  1. 1.Department of Plant PhysiologyAdam Mickiewicz UniversityPoznanPoland

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