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Capillary Electrophoresis of Proteins and Peptides pp 39–75Cite as

Covalent and Noncovalent Labeling Schemes for Near-Infrared Dyes in Capillary Electrophoresis Protein Applications

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 276))

Abstract

Capillary electrophoresis (CE) is experiencing increased use in the field of separation science. Part of its growing popularity of capillary electrophoresis can be attributed to the high efficiency of the separations achievable with the technique, making it an attractive tool for bioanalytical applications. Laser-induced fluorescence (LIF) is a common detection method for CE. One of the problems frequently experienced when using visible LIF detection is matrix autofluorescence which has the effect of degrading the overall sensitivity of the technique. However, the use of near-infrared (NIR) laser induced fluorescence nearly eliminates matrix autofluorescence, as very few molecules have intrinsic fluorescence in this region. This chapter describes the use of covalent and noncovalent labeling schemes for tagging biomolecules with near infrared dyes. To fully appreciate the advantages that the NIR LIF technique can supply, we also review applications that employ detection schemes other than NIR LIF. Specific applications to be discussed include drug-protein studies by CE, as well as capillary electrophoretic immunoassays.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Georgia State University, University Plaza, Atlanta, GA

    John Sowell, Jozef Salon, Lucjan Strekowski & Gabor Patonay

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  1. John Sowell
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  2. Jozef Salon
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  4. Gabor Patonay
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Editor information

Editors and Affiliations

  1. Eli Lilly and Company, Indianapolis, IN

    Mark A. Strege

  2. Lilly Research Laboratories, Indianapolis, IN

    Avinash L. Lagu

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© 2004 Humana Press Inc.

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Sowell, J., Salon, J., Strekowski, L., Patonay, G. (2004). Covalent and Noncovalent Labeling Schemes for Near-Infrared Dyes in Capillary Electrophoresis Protein Applications. In: Strege, M.A., Lagu, A.L. (eds) Capillary Electrophoresis of Proteins and Peptides. Methods in Molecular Biology™, vol 276. Humana Press. https://doi.org/10.1385/1-59259-798-X:039

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  • DOI: https://doi.org/10.1385/1-59259-798-X:039

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-017-5

  • Online ISBN: 978-1-59259-798-7

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