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Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection

  • Jiaqi Wu
  • Xing-Zheng Wu
  • Tiemin Huang
  • Janusz Pawliszyn
Part of the Methods in Molecular Biology™ book series (MIMB, volume 276)

Abstract

The recently developed whole-column-imaging detection technique for capillary electrophoresis (CE) and capillary isoelectric focusing (CIEF), a commercial whole-column-imaged CIEF instrument and its standard operation protocol are introduced. Furthermore, new developments and applications of whole-column-imaging detection in protein-protein interaction study, in protein separation using microfluidic devices and CIEF methods without carrier ampholytes, as well as in 2D separation techniques are reviewed. Miniaturization of whole-column-imaging CIEF and axially illuminated fluorescence whole-column-imaging CIEF are also discussed.

Key Words

Capillary electrophoresis capillary isoelectric focusing conjugation reaction microfluidic device protein whole-column-imaging detection 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Jiaqi Wu
    • 1
  • Xing-Zheng Wu
    • 2
  • Tiemin Huang
    • 3
  • Janusz Pawliszyn
    • 3
  1. 1.Convergent Bioscience Ltd.TorontoCanada
  2. 2.Department of Materials Science and EngineeringFukui UniversityFukui-shiJapan
  3. 3.Department of ChemistryUniversity of WaterlooWaterlooCanada

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