On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detection

  • Hossein Ahmadzadeh
  • Sergey Krylov
Part of the Methods in Molecular Biology™ book series (MIMB, volume 276)


This chapter presents methods for capillary electrophoresis (CE) fingerprinting of proteins in a cell extract and in single cells. A custom-made CE instrument with laser-induced fluorescence (LIF) detection, used for the analyses, is described. Detailed procedures are given for: (1) on column labeling of proteins with a fluorogenic reagent, 5-furoyl quinoline-3- carboxaldehyde, (2) CE separation of labeled proteins, (3) preparation of a protein extract from cultured cells, and (4) manipulations associated with analyses of proteins in single cells. More than 20 relevant publications are cited in this chapter to assist the reader with adopting the presented methods.

Key Words

Capillary electrophoresis labeling laser-induced fluorescence proteins single cell 


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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Hossein Ahmadzadeh
    • 1
  • Sergey Krylov
    • 2
  1. 1.Department of ChemistryUniversity of MinnesotaMinneapolis
  2. 2.Department of ChemistryYork UniversityTorontoCanada

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