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Analysis of B-Cell Life-Span and Homeostasis

  • Irmgard Föster
Part of the Methods in Molecular Biology book series (MIMB, volume 271)

Abstract

In the past, the life-span of B cells in rodents has been determined by a variety of methods, leading to conflicting results. Among the various techniques employed, labeling of dividing cells with the thymidine analog 5-bromo-2′-deoxyuridine (BrdU) has turned out to be a versatile and reliable procedure. Labeling of the cells can be easily performed in vivo by feeding BrdU in the drinking water for extended periods of time or by an ip injection of BrdU for short-term labeling experiments. Using the protocol described, it is possible to combine flow cytometric detection of incorporated BrdU simultaneously with fluorescence staining of various cell surface markers.

Key Words

BrdU B cell life-span flow cytometry proliferation 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Irmgard Föster
    • 1
  1. 1.Institute for Medical Microbiology, Immunology, and Hygiene and Department of Internal Medicine IITechnical University of MunichMunichGermany

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