Study of Tyrosine Kinases and Protein Tyrosine Phosphorylation

  • Jonathan M. Gibbins
Part of the Methods in Molecular Biology™ book series (MIMB, volume 273)

Abstract

In recent years, our increased understanding of the complex signal transduction mechanisms that regulate cellular function has fueled huge advances in all aspects of biomedical science and cell biology. Platelet and megakaryocyte function is no exception to this. In the last 10 yr our understanding of the receptor biochemistry and the systems that they control has been pivotal in the development of new strategies to inhibit platelet function and thereby prevent thrombosis. Experimental techniques have become more and more elegant, however; the basic toolbox that a researcher requires to study signaling in platelets and megakaryoctes is described in this and several subsequent chapters.

Keywords

Vortex Agarose MgCl2 Immobilization Serine 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Jonathan M. Gibbins
    • 1
  1. 1.School of Animal & Microbial SciencesThe University of ReadingReadingUK

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