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In Vivo Models of Platelet Function and Thrombosis

Study of Real-Time Thrombus Formation

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Book cover Platelets and Megakaryocytes

Abstract

Our understanding of hemorrhagic and thrombotic diseases has expanded with use of models aimed at studying the vasculature using a variety of different animals. Unlike in vitro experiments, these animal models enable the study of the broad continuum of biological consequences induced by alterations made to a single variable. This chapter briefly reviews animal models used in the study of thrombosis research, focusing primarily on the use of intravital fluorescence microscopy.

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References

  1. Thackrah, C. T. (1820) An inquiry into the nature and properties of the blood, as existent in health and disease. N. Engl. J Med. Surg. 9, 186–193.

    Google Scholar 

  2. Donne, M. A. (1842) De Forgine des globules du sang, de leur mode de formation et de leur fin. C. R. Acad. Sci. (Paris) 14, 366–368.

    Google Scholar 

  3. Virchow, R. (1856) Uber die Verstopfung der Lungenarterie. Gesammelte Abhandlungen zur wissenschaftlichen Medicin, Frankfort, Meidinger Sohn, p. 221.

    Google Scholar 

  4. Gross, P. L. and Aird, W. C. (2000) The endothelium and thrombosis. Sem. Thromb. Hem. 26, 463–478.

    Article  CAS  Google Scholar 

  5. Rosenblum, W. I. and El-Sabban, F. (1977) Platelet aggregation in the cerebral microcirculation: effect of aspirin and other agents. Circ. Res. 40, 320–328.

    PubMed  CAS  Google Scholar 

  6. Gallagher, K. P., Osakada, G., Kemper, W. S., and Ross., J. (1985) Cyclical coronary flow reductions in conscious dogs equipped with ameroid constrictors to produce severe coronary narrowing. Basic Res. Cardiol. 80, 100–106.

    Article  PubMed  CAS  Google Scholar 

  7. Stockmans, F., Deckmyn, H., Gruwez, J., Vermylen, J., and Acland, R. (1991) Continuous quantitative monitoring of mural, platelet dependent, thrombus kinetics in the crushed rat femoral vein. Thromb. Haemost. 65, 425–431.

    PubMed  CAS  Google Scholar 

  8. Aoki, T., Cox, D., Senzaki, K., Seki, J., Tanaka., A., Takasugi, H., et al. (1998) Comparison of the antithrombotic effects of FK633, GPIlb-IIla antagonist, and aspirin in a guinea pig thrombosis model. Thromb. Res. 89, 129–136.

    Article  PubMed  CAS  Google Scholar 

  9. Kurz, K. D., Main, B. W., and Sandusky, G. E. (1990) Rat model of arterial thrombosis induced by ferric chloride. Thromb. Res. 60, 269–280.

    Article  PubMed  CAS  Google Scholar 

  10. Fay, W. P., Parker, A. C., Ansari, M. N., Zheng, X., and Ginsburg, D. (1999) Vitronectin inhibits the thrombotic response to arterial injury in mice. Blood 93, 1825–1830.

    PubMed  CAS  Google Scholar 

  11. Rosen, E. D., Raymond, S., Zollman, A., Noria, F., Sandoval-Cooper, M., Shulman, A., et al. (2001) Laser-induced noninvasive vascular injury models in mice generate platelet-and coagulation-dependent thrombi. Am. J. Pathol. 158, 1613–1622.

    Article  PubMed  CAS  Google Scholar 

  12. Falati, S., Gross, P., Merrill-Skoloff, G., Furie, B. C., and Furie, B. (2002) Real-time imaging of the assembly of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse. Nat. Med. 8, 1175–1181.

    Article  PubMed  CAS  Google Scholar 

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© 2004 Humana Press Inc.

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Falati, S. et al. (2004). In Vivo Models of Platelet Function and Thrombosis. In: Gibbins, J.M., Mahaut-Smith, M.P. (eds) Platelets and Megakaryocytes. Methods In Molecular Biology™, vol 272. Humana Press. https://doi.org/10.1385/1-59259-782-3:187

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  • DOI: https://doi.org/10.1385/1-59259-782-3:187

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-101-1

  • Online ISBN: 978-1-59259-782-6

  • eBook Packages: Springer Protocols

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