Skip to main content

Platelet Aggregation in Whole Blood

Impedance and Particle Counting Methods

  • Protocol
Platelets and Megakaryocytes

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 272))

  • 2733 Accesses

Abstract

Platelet aggregation and its measurement using the turbidimetric method were discussed in the previous chapter. Many of the same considerations relating to aggregometry that were raised there are equally valid in the context of this chapter and it is recommended that readers acquaint themselves with Chapter 5, vol. 1 prior to commencing this one.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Notes

  1. 1.

    *

    For a sphere:

References

  1. De La Cruz, J. P., Paez, M. V., Carmona, J. A., and De La Cuesta, F. S. (1999) Antiplatelet effect of the anaesthetic drug propofol: influence of red blood cells and leucocytes. Br. J. Pharmacol. 128, 1538–1544.

    Article  Google Scholar 

  2. Jarvis, G. E. and Evans, R. J. (1994) Endotoxin-induced platelet aggregation in heparinised equine whole blood in vitro. Res. Vet. Sci. 57, 317–324.

    Article  PubMed  CAS  Google Scholar 

  3. Heptinstall, S., Fox, S., Crawford, J., and Hawkins, M. (1986) Inhibition of platelet aggregation in whole blood by dipyridamole and aspirin. Thromb. Res. 42, 215–223.

    Article  PubMed  CAS  Google Scholar 

  4. Riess, H., Braun, G., Brehm, G., and Hiller, E. (1986) Critical evaluation of platelet aggregation in whole human blood. Am. J. Clin. Pathol. 85, 50–56.

    PubMed  CAS  Google Scholar 

  5. Sweeney, J. D., Labuzzetta, J. W., Michielson, C. E., and Fitzpatrick, J. E. (1989) Whole blood aggregation using impedance and particle counter methods. Am. J. Clin. Pathol. 92, 794–797.

    PubMed  CAS  Google Scholar 

  6. Cardinal, D. C. and Flower, R. J. (1980) The electronic aggregometer: a novel device for assessing platelet behavior in blood. J. Pharmacol. Methods 3, 135–158.

    Article  PubMed  CAS  Google Scholar 

  7. Gordge, M. P., Dodd, N. J., Rylance, P. B., and Weston, M. J. (1984) An assessment of whole blood impedance aggregometry using blood from normal subjects and haemodialysis patients. Thromb. Res. 36, 17–27.

    Article  PubMed  CAS  Google Scholar 

  8. Bevan, J. and Heptinstall, S. (1985) Serotonin-induced platelet aggregation in whole blood and the effects of ketanserin and mepyramine. Thromb. Res. 38, 189–194.

    Article  PubMed  CAS  Google Scholar 

  9. Fox, S. C., Burgess-Wilson, M., Heptinstall, S., and Mitchell, J. R. (1982) Platelet aggregation in whole blood determined using the Ultra-Flo 100 Platelet Counter. Thromb. Haemost. 48, 327–329.

    PubMed  CAS  Google Scholar 

  10. Storey, R. F., Sanderson, H. M., White, A. E., May, J. A., Cameron, K. E., and Heptinstall, S. (2000) The central role of the P2T receptor in amplification of human platelet activation, aggregation, secretion and procoagulant activity. Br. J. Haematol. 110, 925–934.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2004 Humana Press Inc.

About this protocol

Cite this protocol

Jarvis, G.E. (2004). Platelet Aggregation in Whole Blood. In: Gibbins, J.M., Mahaut-Smith, M.P. (eds) Platelets and Megakaryocytes. Methods In Molecular Biology™, vol 272. Humana Press. https://doi.org/10.1385/1-59259-782-3:077

Download citation

  • DOI: https://doi.org/10.1385/1-59259-782-3:077

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-101-1

  • Online ISBN: 978-1-59259-782-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics