Abstract
Platelets are very small—the smallest of cellular elements in circulating blood. They lack a nucleus and their cytoplasm is relatively clear. As a result it was impossible to visualize platelets in the crude instruments used by early microscopists. In fact, platelets were not identified until many years after red blood cells and leukocytes were well known (1,2). It took the development of the compound and achromatic microscopes (3) to see platelets, and even then they were considered fragments of erythrocytes or white blood cells (4,5). Difficulties in anticoagulating blood and preparing samples for study in microscopes complicated the problem and delayed for decades the realization that platelets were involved in blood clotting and thrombosis (6). That platelets were critical cellular elements for hemostasis in vivo took even longer (7). It was not until the electron microscope became available that insights into relationships among platelet structure, function, and pathology began to evolve (8,9). Even then, the problems of fixing platelets to preserve their fine structure and relate physical changes following activation to their role in hemostatic physiology took considerable time (10).
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References
Lee, P. Y. H. (1988) in pursuit of platelets—the third element of blood. Pharos Alpha Omega Honor Med. Hon. Soc. 51, 8–13.
Robb-Smith, A. H. T. (1967) Why the platelets were discovered. Br. J. Haematol. 13, 618–637.
Clay, R. S. and Court, T. H. (1932) The History of the Microscope. Charles Griffin & Co., London, p. 20.
Donné, A. (1842) De l’origine des globules du sang, de leur mode de formation et de leur fin. CR Seances Acad. Sci. 14, 366–368.
Howell, W. H. (1884) The new morphological element of the blood. Science 3, 46–49.
Bizzozero, J. (1882) Uber einen formestandtheil de saugethierblutes und die bedeutung derselben fur die thrombose und blutgerinnung uberhaupt. Centralbl. Med. Wissensch. 20, 17–20.
Bizzozero, J. (1983) D’un nouvel element morphologique de sang et de son importance dans la thrombose et dans la coagulation. Arch. Ital. Biol. 2, 345–362; (1982) 3, 94–121.
Wolpers, C. and Ruska, H. (1939) Stukturuntersuchungen zur blutgerinnung. Klinische Wochenschrift 23, 1077–1081; 1111–1117.
David-Ferreira, J. F. (1964) The blood platelet: electron microscopic studies. Int. Rev. Cytol. 55, 89–103.
Rebuck, J. W., Riddle, J. M., Johnson, S. A., Monto, R. W., and Sturrock, R. M. (1959) Contributions of electron microscopy to the study of blood platelets. Proceeding of the Third Conference on Platelets. Detroit, MI, pp. 2–22.
Sabatini, D. D., Bensch, K. G., and Barnett, R. S. (1962) Preservation of ultrastructure and enzymatic activity by aldehyde fixation. Anatom. Rec. 142, 274–283.
White, J. G. and Krivit, W. (1965) Fine structural localization of adenosine triphosphosphatase in human platelets and other blood cells. Blood 26, 554–568.
White, J. G. (1971) Platelet morphology. In The Circulating Platelet (Johnson, S. A., ed.), Academic Press, New York, pp. 45–121.
White, J. G. (1971) The ultrastructural cytochemistry and physiology of blood platelets. In The Platelet (Mostafi, F. K. and Brinkhous, K. M., eds.), Williams and Wilkins, Baltimore, MD, pp. 83–115.
Wood, J. G. (1965) Electron microscopic localization of 5-hydroxytryptamine (5-HT). Texas Reports on Biology and Medicine 23, 828–837.
Behnke, O. (1996) Incomplete microtubules observed in mammalian blood platelets during microtubule polymerization. J. Cell Biol. 34, 697–701.
White, J. G. and Krivit, W. (1967) An ultrastructural basis for the shape changes induced by chilling. Blood 30, 625–635.
Tang, S. S. and Frojmovic, M. M. (1977) The effects of pCO2 and pH on drug-induced platelet sphering and aggregation for human and rabbit platelet-rich plasma. Thromb. Res. 10, 135–145.
O’Brien, J. R. and Woodhouse, M. A. (1968) Platelets: their size, shape and stickiness in citro: degranulation and propinquity. Exper. Biol. Med. Karger, Basel 3, 90–102.
White, J. G. (1968) Fine structural alterations induced in platelets by adenosine diphosphate. Blood 31, 604–622.
Karnovsky, M. J. (1971) Use of ferrocyanide reduced osmium terroxide in electron microscopy. American Society of Cell Bioliogy, Annual Meeting, New Orleans.
White, J. G. (1971) Platelet Morphology. In The Circulating Platelet (Johnson, S. E., ed.), Academic Press, New York, 45–121.
White, J. G. (1971) The ultrastructural cytochemistry and physiology of blood platelets. In The Platelet (Mostafi, F. K. and Brinkhous, K. M., eds.), Williams and Wilkins, Baltimore, MD, 873–915.
Breton-Gorius, J. and Guichard, J. (1975) Two different types of granules in megakaryocytes and platelets as revealed by the diaminobenzidine reaction. J. Micros. Biol. 23, 197–202.
Bentfield, M. E. and Bainton, D. F. (1975) Cytochemical localization of lysosomal enzymes in rat megakaryocytes and platelets. J. Clin. Invest. 56, 1635–1639.
White, J. G. (1969) The dense bodies of human platelets: inherent electron opacity of serotonin storage organelles. Blood 33, 598–606.
White, J. G. and Escolar, G. (1993) Current concepts of platelet membrane response to surface activation. Platelets 4, 176–189.
White, J. G. and Conard, W. J. (1973) The fine structure of freeze-fractured blood platelets. Am. J. Pathol. 70, 45–56.
White, J. G. and Clawson, C. C. (1980) Biostructure of blood platelets. Ultrastruct. Pathol. 1, 533.
White, J. G. and Clawson, C. C. (1980) The surface-connected canalicular system of blood platelets-A fenestrated membrane system. Am. J. Pathol. 101, 353.
Breton-Gorius, J. and Guichard, J. (1972) Ultrastructural localization of peroxidase activity in human platelets and megakaryocytes. Am. J. Pathol. 66, 277–286.
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White, J.G. (2004). Electron Microscopy Methods for Studying Platelet Structure and Function. In: Gibbins, J.M., Mahaut-Smith, M.P. (eds) Platelets and Megakaryocytes. Methods In Molecular Biology™, vol 272. Humana Press. https://doi.org/10.1385/1-59259-782-3:047
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DOI: https://doi.org/10.1385/1-59259-782-3:047
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