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Serial Analyses of Gene Expression (SAGE)

  • Jia Le Dai
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 103)

Abstract

Serial analysis of gene expression (SAGE) is a molecular biology technique that was developed to measure the global gene expression levels. It has been applied successfully to characterize transcriptomes, compare the transcript levels between normal and diseased tissues, and uncover novel molecules within defined signal transduction pathways. A detailed description is presented in this chapter of the procedures involved to prepare the SAGE libraries. Protocols for automated sequencing and other standard molecular biology techniques can be found elsewhere, and thus are not included herein.

Key Words

Serial analysis of gene expression tags ditags anchoring enzyme tagging enzyme cDNA 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Jia Le Dai
    • 1
  1. 1.Department of Molecular Pathology, M. D. Anderson Cancer CenterUniversity of TexasHouston

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