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Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis

  • Kimberly Walter
  • James Eshleman
  • Michael Goggins
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 103)

Abstract

Xenotransplantation (xenografting) of primary cancers or cancer cell lines into immunodeficient mice is a commonly used technique to assess tumor growth in response to a variety of experimental agents. When primary pancreatic cancers are xenografted, cancer cells proliferate in the mouse, but the human stroma does not. This growth pattern enables facile genetic analysis of cancer genetics profiles without the contamination of admixed stromal cells typical of primary cancers.

Key Words

DNA pancreatic cancer xenograft 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Kimberly Walter
    • 1
  • James Eshleman
    • 1
    • 2
    • 3
  • Michael Goggins
    • 1
    • 2
    • 3
  1. 1.Department of PathologyThe Johns Hopkins University School of MedicineBaltimore
  2. 2.Department of MedicineThe Johns Hopkins University School of MedicineBaltimore
  3. 3.The Oncology CenterThe Johns Hopkins University School of MedicineBaltimore

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