In Vitro RNA Editing Systems From Higher Plant Chloroplasts

Part of the Methods in Molecular Biology book series (MIMB, volume 265)


RNA editing in higher plant chloroplasts involves C-to-U conversion at ∼30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates labeled with 32P at C residues to be edited provide sensitive detection of editing activity in vitro. The present systems allow the rapid identification of cis-elements using mutated mRNA substrates and trans-acting factors by ultraviolet crosslinking.

Key Words

Chloroplast cis-acting element in vitro system pea RNA editing RNA ligation site-specific labeling tobacco trans-acting factor 


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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  1. 1.School of Biomedical ScienceTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Center for Gene ResearchNagoya UniversityNagoyaJapan
  3. 3.Graduate School of Natural SciencesNagoya City UniversityNagoyaJapan

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