Abstract
RNA editing in higher plant chloroplasts involves C-to-U conversion at ∼30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates labeled with 32P at C residues to be edited provide sensitive detection of editing activity in vitro. The present systems allow the rapid identification of cis-elements using mutated mRNA substrates and trans-acting factors by ultraviolet crosslinking.
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Hirose, T., Miyamoto, T., Obokata, J., Sugiura, M. (2004). In Vitro RNA Editing Systems From Higher Plant Chloroplasts. In: Gott, J.M. (eds) RNA Interference, Editing, and Modification. Methods in Molecular Biology, vol 265. Humana Press. https://doi.org/10.1385/1-59259-775-0:333
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DOI: https://doi.org/10.1385/1-59259-775-0:333
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