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Identification and Characterization of Trypanosome RNA-Editing Complex Components

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Part of the Methods in Molecular Biology book series (MIMB, volume 265)

Abstract

This chapter describes the methods used to purify the RNA-editing complex, to identify the proteins by mass spectrometry, and to demonstrate the functions for some of the proteins.

Key Words

Chromatography editosome mass spectrometry monoclonal antibodies protein RNA editing tandem affinity purification tag 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  1. 1.Seattle Biomedical Research InstituteUniversity of WashingtonSeattle
  2. 2.Department of PathobiologyUniversity of WashingtonSeattle

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