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In Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated Activities

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RNA Interference, Editing, and Modification

Part of the book series: Methods in Molecular Biology ((MIMB,volume 265))

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Abstract

This chapter describes biochemical assays that have been used in analyzing RNA editing in kinetoplastid mitochondria and to characterize the general mechanism of editing by the editosome. Studies using these assays have shown that the characteristics of each activity contribute to editing site selection, U addition and removal, and RNA ligation resulting in accurately edited mRNAs.

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Author information

Authors and Affiliations

  1. Seattle Biomedical Research Institute, University of Washington, Seattle, WA

    Kenneth Stuart, Reza Salavati, Nancy Lewis Ernst, Setareh S. Palazzo & Bingbing Wang

  2. Department of Pathobiology, University of Washington, Seattle, WA

    Kenneth Stuart, Reza Salavati, Nancy Lewis Ernst, Setareh S. Palazzo & Bingbing Wang

  3. Department of Biostatistics, University of Washington, Seattle, WA

    Robert P. Igo Jr.

Authors
  1. Kenneth Stuart
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  2. Reza Salavati
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  3. Robert P. Igo Jr.
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  4. Nancy Lewis Ernst
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  5. Setareh S. Palazzo
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  6. Bingbing Wang
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Editor information

Editors and Affiliations

  1. Case Western Reserve University School of Medicine, Cleveland, OH

    Jonatha M. Gott

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© 2004 Humana Press Inc.

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Stuart, K., Salavati, R., Igo, R.P., Ernst, N.L., Palazzo, S.S., Wang, B. (2004). In Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated Activities. In: Gott, J.M. (eds) RNA Interference, Editing, and Modification. Methods in Molecular Biology, vol 265. Humana Press. https://doi.org/10.1385/1-59259-775-0:251

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  • DOI: https://doi.org/10.1385/1-59259-775-0:251

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-242-1

  • Online ISBN: 978-1-59259-775-8

  • eBook Packages: Springer Protocols

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