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Methods for Delivery of Double-Stranded RNA into Caenorhabditis elegans

Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 265)

Abstract

The nematode Caenorhabditis elegans is often employed in investigations of diverse aspects of biology, including behavior, development, basic cellular processes, and disease states. The ability to utilize double-stranded RNA (dsRNA) to inhibit specific gene function in this organism has dramatically increased its value for these kinds of studies and has provided more flexibility in experimental design that include procedures. Here, we have collected a set of protocols from the C. elegans community for propagation of C. elegans, techniques for dsRNA preparation, four basic methods for delivery of dsRNA to C. elegans (injection, soaking, feeding, and in vivo delivery), and we suggest schemes that should facilitate detection of specific gene silencing.

Key Words

Double-stranded RNA single-stranded RNA RNA silencing feeding soaking injection HT115 HT115(DE3) transgene transcription RNAi 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  1. 1.Department of Molecular BiosciencesUniversity of KansasLawrence

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