Differentiation of Lactic Acid Bacteria Strains by Postelectrophoretic Detection of Esterases

  • Roxana Beatriz Medina
  • Marta Beatriz Katz
  • Silvia González
Part of the Methods in Molecular Biology book series (MIMB, volume 268)


Lactic acid bacteria (LAB) comprise a diverse group of Gram-positive, non-sporeforming microorganisms. These bacteria are widely used in food technology. The species identification of LAB depends mainly on physiological and biochemical criteria. The esterolytic systems of LAB remain poorly characterized. Esterases (EC represent a diverse group of hydrolases catalyzing the cleavage and formation of esters bonds (1) Screening of esterases is usually performed either by employing chromophoric substances (e.g., α- or β-naphthyl esters of short-chain fatty acids). The post-electrophoretic detection of esterases is a sensitive technique applied in bacterial systems, that mainly provides information on the similarity of strains within the same species or subspecies according to their esterase patterns. This technique is principally used to determine the number and substrate specificity of esterases and lipases, revealing the complexity of lipase and esterase systems (2,3). The present chapter describes the technique of polyacrylamide gel electrophoresis (PAGE; in the absence of sodium dodecyl sulfate [SDS]), in non-denaturing conditions, to find intracellular fractions for strain typing of LAB.


Lactic Acid Bacterium Lactic Acid Bacterium Strain Intracellular Fraction Reservoir Buffer Acrylamide Solution 
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  1. 1.
    Bornscheuer, U. T. (2002) Microbial carboxyl esterases: classification, properties and application in biocatalysis. FEMS Microbiol. Rev. 26, 73–81.PubMedCrossRefGoogle Scholar
  2. 2.
    Tsakalidou, E., Zoidou, E., and Kalantzopoulos, G. (1992) Esterase activities of cell-free extracts from strains of Lactococcus lactis subsp. lactis isolated from traditional Greek cheese. J. Dairy Res. 59, 111–113.CrossRefGoogle Scholar
  3. 3.
    Vafopoulou-Mastrojiannaki, A., Litopoulou-Tzanetaki, E., and Tzanetakis, N. (1996) Esterase activities of cell-free extracts from wild strains of leuconostoc and heterofermentative lactobacilli isolated from traditional Greek cheese. Lett. Appl. Microbiol. 23, 367–370.CrossRefGoogle Scholar
  4. 4.
    De Man, J. C., Rogosa, M., and Sharpe, M. E. (1960) A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23, 130–135.Google Scholar
  5. 5.
    Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277, 680–685.CrossRefGoogle Scholar
  6. 6.
    Morichi, T., Sharpe, M. E., and Reiter, D. (1968). Esterase and other soluble proteins of some lactic acid bacteria. J. Gen. Microbiol. 53, 405–414PubMedGoogle Scholar
  7. 7.
    Katz, M., Medina, R., Gonzalez, S., and Oliver, G. (2002) Esterolytic and lipolytic activities of lactic acid bacteria isolated from ewe’s milk and cheese. J. Food Prot. 65, 1997–2001.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. Totowa, NJ 2004

Authors and Affiliations

  • Roxana Beatriz Medina
    • 1
    • 2
  • Marta Beatriz Katz
    • 1
  • Silvia González
    • 1
    • 2
  1. 1.Centro de Referencia para Lactobacilos (CERELA)-CONICETTucumánArgentina
  2. 2.Universidad Nacional de TucumánTucumánArgentina

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