Abstract
Denaturing gradient gel electrophoresis (DGGE) has been developed as a technique to screen for mutations in a particular gene. Usually, PCR products of a particular gene in eukaryotic cells, prokaryotic cells, or clones (e.g., wild-type strain and a mutant) are amplified, and separated on a gel in which a gradient of increasing chemical denaturant is established. Under optimal conditions, PCR products that have nucleotide substitutions will migrate to a different position on DGGE in comparison to the original PCR products, and thus allow the detection of mutations in this particular gene.
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Baker, P.W., Harayama, S. (2004). An Analysis of Microorganisms in Environments Using Denaturing Gradient Gel Electrophoresis. In: Walker, J.M., Spencer, J.F.T., Ragout de Spencer, A.L. (eds) Environmental Microbiology. Methods in Biotechnology, vol 16. Humana Press. https://doi.org/10.1385/1-59259-765-3:323
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DOI: https://doi.org/10.1385/1-59259-765-3:323
Publisher Name: Humana Press
Print ISBN: 978-1-58829-116-5
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