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PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of Environmental Bacteria Isolates

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Environmental Microbiology

Part of the book series: Methods in Biotechnology ((MIBT,volume 16))

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Abstract

The analysis of DNA or RNA molecules has been used in a large number of studies on bacterial taxonomy and typing and for diversity studies. Undoubtedly, these methods presently dominate modern taxonomic studies as a consequence of technological progress, but primarily because the present view on classification is that it should reflect the natural relationships as encoded in the DNA. Some of these methods are not fully sequence-based, although in a number of cases they have provided considerably more information than traditional phenotypic methods (1).

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Martínez, M.A., Siñeriz, F. (2004). PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of Environmental Bacteria Isolates. In: Walker, J.M., Spencer, J.F.T., Ragout de Spencer, A.L. (eds) Environmental Microbiology. Methods in Biotechnology, vol 16. Humana Press. https://doi.org/10.1385/1-59259-765-3:159

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  • DOI: https://doi.org/10.1385/1-59259-765-3:159

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-116-5

  • Online ISBN: 978-1-59259-765-9

  • eBook Packages: Springer Protocols

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