Abstract
The ability to track or monitor individual bacterial cells in a given system has the potential to yield new data about the factors affecting the survival, movement, and growth of bacteria in a variety of environments and under a range of conditions. For field-scale studies, the methods have mainly focused on the use of nucleic-acid-binding fluorescent stains, most of which have detrimental effects on cell properties and functions, and also pose a health risk due to their inherent toxicity.
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References
Haugland, R. (1996) Handbook of Fluorescent Probes and Research Chemicals, sixth edition (Spence, M., ed). Molecular Probes, Inc., Eugene, OR.
Hareland, W. A., Crawford, R. L., Chapman, P. J., and Dagley, S. (1975) Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans. J. Bacteriol. 121, 272–285.
Fuller, M. E., Mailloux, B. J., Zhang, P., Streger, S. H., Hall, J. A., Vainberg, S. N., et al. (2001) Field-scale evaluation of CFDA/SE staining coupled with multiple detection methods for assessing the transport of bacteria in situ. FEMS Microbiol. Ecol. 37, 55–66.
Fuller, M. E., Streger, S. H., Rothmel, R. K., Mailloux, B. J., Onstott, T. C., Fredrickson, J. K., et al. (2000) Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments. Appl. Environ. Microbiol. 66, 4486–4496.
Johnson, W. P., Zhang, P., Fuller, M. E., Scheibe, T., Mailloux, B. J., Onstott, T. C., et al. (2001) Ferrographic tracking of bacterial transport in the field at Oyster, VA. Environ. Sci. Technol. 35, 182–191.
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Fuller, M.E. (2004). Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in Subsurface Ground Water. In: Walker, J.M., Spencer, J.F.T., Ragout de Spencer, A.L. (eds) Environmental Microbiology. Methods in Biotechnology, vol 16. Humana Press. https://doi.org/10.1385/1-59259-765-3:103
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DOI: https://doi.org/10.1385/1-59259-765-3:103
Publisher Name: Humana Press
Print ISBN: 978-1-58829-116-5
Online ISBN: 978-1-59259-765-9
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