Abstract
A wide diversity of microorganisms, including bacteria, archaea, fungi, and microalgae, are found in marine habitats. Although there has been much progress in describing the diversity of marine bacteria and archaea, we still know very little about the “role” of these microbes (1).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Kirchman, D. L. and Williams, P. J. leB. (2000) Introduction, in Microbial Ecology of the Oceans (Kirchman, D. L., ed.), John Wiley & Sons, Inc., New York, pp. 1–12.
Jannasch, H. W. and Jones, G. E. (1959) Bacterial populations in seawater as determined by different methods of enumeration. Limnol. Oceanogr. 4, 128–139.
Sherr, E. and Sherr, B. (2000) Marine microbes. An overview, in Microbial Ecology of the Oceans (Kirchman, D. L., ed.), John Wiley & Sons, Inc., New York, pp. 13–46.
Weidner, S., Arnold, W., and Puhler, A. (1996) Diversity of uncultured microorganisms associated with the seagrass Halophila stipulacea estimated by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes. Appl. Environ. Microbiol. 62, 766–771.
Connon, S. A. and Giovannoni, S. J. (2002) High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl. Environ. Microbiol. 68, 3878–3885.
Eilers, H., Pernthaler, J., Glöckner, F. O., and Amann, R. (2000) Culturability and in situ abundance of pelagic bacteria from the North Sea. Appl. Environ. Microbiol. 66, 3044–3051.
Button, D. K., Schut, F., Quang, P., Martin, R., and Robertson, B. R. (1993) Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results. Appl. Environ. Microbiol. 59, 1707–1713.
Woese, C. R. (1987) Bacterial evolution. Microbiol. Rev. 51, 221–271.
Stackebrandt, D. and Goebel, B. M. (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Sys. Bacteriol. 44, 846–849.
Gurtler, V. and Stanisich V. A. (1996) New approaches to typing and identification of bacteria using the 16S–23S rDNA spacer region. Microbiology 142, 3–16.
Suzuki, M. T., Rappé, M. S., Zara, W. H., Winfield, H., Adair, N., Strobel, J., et al. (1997) Bacterial diversity among small-subunit rRNA gene clones and cellular isolates from the same seawater sample. App. Environ. Microbiol. 63, 983–989.
Sambrook, J., Fristch, E. F., and Maniatis, T. (eds.) (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Lane, D. J. (1991) 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics (Stackebrandt, E. and Goodfellow, M., eds.). John Wiley & Sons, Inc., New York.
Daffonchio, D., Borin, S., Frova, G., and Sorlini, C. (1998) PCR fingerprinting of whole genomes: the spacer between the 16S and 23S rRNA genes and of intergenic tRNA gene regions reveals a different intraspecific genomic variability of Bacillus cereus and Bacillus licheniformis. Int. J. Syst. Bacteriol. 48, 107–116.
Ferguson, R. L., Buckley, E. N., and Palumbo, A. V. (1984) Response of marine bacterioplankton to differential filtration and confinement. Appl. Environ. Microbiol. 47, 49–55.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc.
About this protocol
Cite this protocol
Benito, J.M., Lovrich, G.A., Siñeriz, F., Abate, C.M. (2004). Isolation and Molecular Characterization of Seawater Bacteria. In: Walker, J.M., Spencer, J.F.T., Ragout de Spencer, A.L. (eds) Environmental Microbiology. Methods in Biotechnology, vol 16. Humana Press. https://doi.org/10.1385/1-59259-765-3:003
Download citation
DOI: https://doi.org/10.1385/1-59259-765-3:003
Publisher Name: Humana Press
Print ISBN: 978-1-58829-116-5
Online ISBN: 978-1-59259-765-9
eBook Packages: Springer Protocols