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Genomics, Proteomics, and Clinical Bacteriology pp 191–211Cite as

Real-Time PCR

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 266))

Abstract

The development of instruments that allow real-time monitoring of fluorescence within PCR reaction vessels is a significant advance in clinical bacteriology. The technology is very flexible, and many alternative instruments and fluorescent probe systems are currently available. Real-time PCR assays can be completed very rapidly, because no manipulations are required after amplification. Identification of amplification products by probe detection in real time is highly accurate compared with size analysis on gels. Analysis of the progress of the reaction allows accurate quantification of the target sequence over a very wide dynamic range, provided suitable standards are available. Finally, probe melting analysis can detect sequence variants including single base mutations.

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Author information

Authors and Affiliations

  1. Genomics, Proteomics, and Bioinformatics Unit, Specialist and Reference Microbiology Division, Health Protection Agency-Colindale, London, UK

    Nicholas A. Saunders

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  1. Nicholas A. Saunders
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Editor information

Editors and Affiliations

  1. Antibiotic Resistance Monitoring and Reference Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency-Colindale, London, UK

    Neil Woodford  & Alan P. Johnson  & 

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© 2004 Humana Press Inc.

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Saunders, N.A. (2004). Real-Time PCR. In: Woodford, N., Johnson, A.P. (eds) Genomics, Proteomics, and Clinical Bacteriology. Methods in Molecular Biology™, vol 266. Humana Press. https://doi.org/10.1385/1-59259-763-7:191

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  • DOI: https://doi.org/10.1385/1-59259-763-7:191

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-218-6

  • Online ISBN: 978-1-59259-763-5

  • eBook Packages: Springer Protocols

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