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Exploring and Exploiting Bacterial Proteomes

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Book cover Genomics, Proteomics, and Clinical Bacteriology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 266))

Abstract

The plethora of data now available from bacterial genome sequencing has opened a wealth of new research opportunities. Many of these have been reviewed in preceding chapters. Genomics alone, however, cannot capture a biological snapshot from an organism at a given point in time. The genome itself is static, and it is the changes in expression of genes, leading to the production of functional proteins, which allows an organism to survive and adapt to a constantly changing environment. Proteomics is the term used to describe the global analysis of proteins involved in a particular biological process. Such processes may be analyzed via comparative studies that examine bacterial strain differences, both phenotypic and genetic, bacteria grown under nutrient limiting conditions, growth phase, temperature, or in the presence of chemical compounds, such as antibiotics. Proteomics also provides the researcher with a tool to begin characterizing the functions of the vast proportion of “hypothetical” or “unknown” proteins elucidated from genome sequencing and database comparisons. For example, study of protein-protein, protein-ligand, protein-substrate, and protein-nucleic acid interactions for a given target protein may all help to define the functions of previously unknown proteins. Furthermore, genetic manipulation combined with proteomics technologies can provide an understanding of how gene expression is regulated. This chapter examines the technologies used in proteome analysis and the applications of proteomics to microbiological research, with an emphasis on clinically-relevant bacteria.

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Authors and Affiliations

  1. Australian Proteome Analysis Facility, Macquarie University, Sydney, Australia

    Stuart J. Cordwell

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  1. Stuart J. Cordwell
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Editors and Affiliations

  1. Antibiotic Resistance Monitoring and Reference Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency-Colindale, London, UK

    Neil Woodford  & Alan P. Johnson  & 

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Cordwell, S.J. (2004). Exploring and Exploiting Bacterial Proteomes. In: Woodford, N., Johnson, A.P. (eds) Genomics, Proteomics, and Clinical Bacteriology. Methods in Molecular Biology™, vol 266. Humana Press. https://doi.org/10.1385/1-59259-763-7:115

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  • DOI: https://doi.org/10.1385/1-59259-763-7:115

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-218-6

  • Online ISBN: 978-1-59259-763-5

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