PCR Diagnosis of T-Cell Lymphoma in Paraffin-Embedded Bone Marrow Biopsies

  • Jean Benhattar
  • Sandra Gebhard
Part of the Methods in Molecular Medicine book series (MIMM, volume 97)


Management of T-cell lymphoma patients requires bone marrow examination for the assessment of stage and prognosis (1,2). Bone marrow biopsy (BMB) is mandatory when bone marrow aspirate is not successful (e.g., in cases of patchy lymphomatous infiltration or induction of fibrosis [“dry aspirate”]) (3). Moreover, BMB has some advantages over aspirate, such as examination of a greater volume of tissue with preserved architecture, assessment of cellularity, and application of immunohistochemistry.


Bone Marrow Biopsy Bone Marrow Biopsy Specimen Generate Polymerase Chain Reaction Product Polymerase Chain Reaction Diagnosis Preserve Architecture 
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  1. 1.
    Ansell, S. M., Habermann, T. M., Kurtin, P. J., et al. (1997) Predictive capacity of the international prognostic factor index in patients with peripheral T-cell lymphoma. J. Clin. Oncol. 15, 2296–2301.PubMedGoogle Scholar
  2. 2.
    Caulet, S., Delmer, A., Audouin, J., et al. (1990) Histopathological study of bone marrow biopsies in 30 cases of T-cell lymphoma with clinical, biological and survival correlations. Hematol. Oncol. 8, 155–168.PubMedCrossRefGoogle Scholar
  3. 3.
    Bain, B. J., Clark, D. M., and Lampert, I. A. (1996) In: Bone Marrow Pathology, 2nd ed., (Bain, B. J., ed.), Blackwell Science, London, pp. 1–50.Google Scholar
  4. 4.
    Jaffe, E. S. (1990) The role of immunophenotypic markers in the classification on non-Hodgkin’s lymphomas. Semin. Oncol. 17, 11–19.PubMedGoogle Scholar
  5. 5.
    Griesser, H. (1995) Gene rearrangements and chromosomal translocations in T cell lymphoma—diagnostic applications and their limits. Virchows Arch. 426, 323–338.PubMedCrossRefGoogle Scholar
  6. 6.
    Abbas, A. K., Lichtman, A. H., and Pober, J. S. (1994) In: Cellular and Molecular Immunology, 2nd ed., (Abbas, A. K., ed.), Saunders, Philadelphia, pp. 166–186.Google Scholar
  7. 7.
    Benhattar, J., Delacrétaz, F., Martin, P., et al. (1995) Improved polymerase chain reaction detection of clonal T-cell lymphoid neoplasms. Diagn. Mol. Pathol. 4, 108–112.PubMedCrossRefGoogle Scholar
  8. 8.
    Diss, T. C., Watts, M., Pan, L. X., et al. (1995) The polymerase chain reaction in the demonstration of monoclonality in T cell lymphomas. J. Clin. Pathol. 48, 1045–1050.PubMedCrossRefGoogle Scholar
  9. 9.
    Weirich, G., Funk, A., Hoepner, I., et al. (1995) PCR-based assays for detection of monoclonality in non-Hogkin’s lymphoma: application to formalin-fixed, paraffin-embedded tissue and decalcified bone marrow samples. J. Clin. Med. 73, 235–241.Google Scholar
  10. 10.
    Lorenz, J., Jux, G., Zhao-Hohn, M., et al. (1994) Detection of T-cell clonality in paraffin-embedded tissues. Diagn. Mol. Pathol. 3, 93–99.CrossRefGoogle Scholar
  11. 11.
    Chhanabhai, M., Adomat, S., Gascoyne, R., et al. (1997) Clinical utility of hetero-duplex analysis of TCR gamma gene rearrangements in the diagnosis of T-cell lymphoproliferative disorders. Am. J. Clin. Pathol. 108, 295–301.PubMedGoogle Scholar
  12. 12.
    Signoretti, S., Murphy, M., Cangi, M., et al. (1999) Detection of clonal T-cell receptor γ gene rearrangements in paraffin-embedded tissue by polymerase chain reaction and non-radioactive single-strand conformation polymorphism analysis. Am. J. Pathol. 154, 67–75.PubMedCrossRefGoogle Scholar
  13. 13.
    Gebhard, S., Benhattar, J., Bricod, C., et al. (2001) Polymerase chain reaction in the diagnosis of T-cell lymphoma in paraffin-embedded bone marrow biopsies: a comparative study. Histopathology 38, 37–44.PubMedCrossRefGoogle Scholar
  14. 14.
    Mies, C. (1992) Molecular pathology of paraffin-embedded tissue. Diagn. Mol. Pathol. 1, 206–211.PubMedGoogle Scholar
  15. 15.
    Coad, J. E., Olson, D. J., Christensen, D. R., et al. (1997) Correlation of PCR-detected clonal gene rearrangements with bone marrow morphology in patients with B-lineage lymphomas. Am. J. Pathol. 21, 1047–1056.CrossRefGoogle Scholar
  16. 16.
    Scholte, G., van Doorn, L., Quint, W., et al. (1997) Polymerase chain reaction for the detection of Helicobacter pylori in formaldehyde-sublimate fixed, parrafin-embedded gastric biopsies. Diagn. Mol. Pathol. 6, 238–243.PubMedCrossRefGoogle Scholar
  17. 17.
    Malik, U. R., Oleksowicz, L., Dutcher, J. R., et al. (1996) Atypical clonal T-cell proliferation in infectious mononucleosis. Med. Oncol. 13, 207–213.PubMedCrossRefGoogle Scholar
  18. 18.
    Posnett, D., Sinha, R., Kabak, S., et al. (1994) Clonal populations of T cells in normal elderly humans: the T cell equivalent to “benign monoclonal gammapathy.” J. Exp. Med. 179, 609–618.PubMedCrossRefGoogle Scholar
  19. 19.
    Krafft, A. E., Taubenberger, J. K., Sheng, Z. M., et al. (1999) Enhanced sensitivity with a novel TCR gamma PCR assay for clonality studies 569 formalin-fixed, paraffin-embedded (FFPE) cases. Mol. Diagn. 4, 119–133.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Jean Benhattar
    • 1
  • Sandra Gebhard
    • 1
  1. 1.Institute of PathologyCHUVLausanneSwitzerland

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