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Protein Arrays pp 233-243 | Cite as

Fabrication and Application of G Protein-Coupled Receptor Microarrays

  • Ye Fang
  • Brian Webb
  • Yulong Hong
  • Ann Ferrie
  • Fang Lai
  • Anthony G. Frutos
  • Joydeep Lahiri
Part of the Methods in Molecular Biology book series (MIMB, volume 264)

Abstract

The increased number of drug targets and compounds demands novel high-throughput screening technologies that could be used for parallel analysis of many genes and proteins. Protein microarrays are evolving promising technologies for the parallel analysis of many proteins with respect to their abundance, location, modifications, and interactions with other biological and chemical molecules. This chapter specifically describes the fabrication of G protein-coupled receptor (GPCR) microarrays, a unique subset of protein microarrays, using contact-pin printing technology. The bioassays and potential applications of GPCR microarrays for the determination of compound affinities and potencies are also included.

Key Words

G protein-coupled receptor microarrays protein microarrays G protein-coupled receptor contact-pin printing saturation assay competitive binding assay 

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Ye Fang
    • 1
  • Brian Webb
    • 1
  • Yulong Hong
    • 1
  • Ann Ferrie
    • 1
  • Fang Lai
    • 1
  • Anthony G. Frutos
    • 1
  • Joydeep Lahiri
    • 1
  1. 1.Science and Technology Division, Biochemical TechnologiesCorning Inc.Corning

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